Search results for "Collagen Type X"

showing 2 items of 2 documents

Temozolomide- and fotemustine-induced apoptosis in human malignant melanoma cells: response related to MGMT, MMR, DSBs, and p53

2009

Malignant melanomas are highly resistant to chemotherapy. First-line chemotherapeutics used in melanoma therapy are the methylating agents dacarbazine (DTIC) and temozolomide (TMZ) and the chloroethylating agents BCNU and fotemustine. Here, we determined the mode of cell death in 11 melanoma cell lines upon exposure to TMZ and fotemustine. We show for the first time that TMZ induces apoptosis in melanoma cells, using therapeutic doses. For both TMZ and fotemustine apoptosis is the dominant mode of cell death. The contribution of necrosis to total cell death varied between 10 and 40%. The O(6)-methylguanine-DNA methyltransferase (MGMT) activity in the cell lines was between 0 and 1100 fmol m…

Cancer ResearchProgrammed cell deathDNA repairDacarbazineBlotting WesternApoptosistemozolomideBiologyCollagen Type XIDNA Mismatch RepairNecrosisGliomaAntineoplastic Combined Chemotherapy ProtocolsTumor Cells CulturedmedicineHumansDNA Breaks Double-StrandedEverolimusPhosphorylationDNA Modification MethylasesMelanomaneoplasmsSirolimusTemozolomideTumor Suppressor ProteinsMelanomafotemustinemelanoma therapymedicine.diseaseDacarbazineEnzyme Activationmismatch repairDNA Repair EnzymesOncologyApoptosisCaspasesCancer researchFotemustineTumor Suppressor Protein p53Translational TherapeuticsMGMTmedicine.drugBritish Journal of Cancer
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Human nasoseptal chondrocytes maintain their differentiated phenotype on PLLA scaffolds produced by thermally induced phase separation and supplement…

2018

Damage of hyaline cartilage such as nasoseptal cartilage requires proper reconstruction, which remains challenging due to its low intrinsic repair capacity. Implantation of autologous chondrocytes in combination with a biomimetic biomaterial represents a promising strategy to support cartilage repair. Despite so far mostly tested for bone tissue engineering, bioactive glass (BG) could exert stimulatory effects on chondrogenesis. The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes. The PLLA scaffolds without or…

Malecartilage tissue engineering02 engineering and technologyBiochemistrylaw.inventionExtracellular matrixX-Ray DiffractionlawOrthopedics and Sports MedicineGlycosaminoglycansExtracellular Matrix Proteins0303 health sciencesSettore ING-IND/24 - Principi Di Ingegneria ChimicaCalorimetry Differential ScanningTissue ScaffoldsChemistryHyaline cartilageTemperatureSettore ING-IND/34 - Bioingegneria IndustrialeCell DifferentiationMiddle AgedPhenotypemedicine.anatomical_structureBioactive glassFemaleAdultPolyesters0206 medical engineeringType II collagenNoseChondrocyteYoung Adult03 medical and health sciencesChondrocytesRheumatologymedicineHumanspoly(L)lactic acidCollagen Type IIMolecular BiologyAggrecan030304 developmental biologyCartilagenasoseptal chondrocyteCell BiologyChondrogenesis020601 biomedical engineeringBioactive glass 1393Gene Expression RegulationBiophysicschondrogenesiGlassCollagen Type X
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